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CABRI:ICLC |
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Accession_number |
ICLC HTL14002
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Cell_line_name |
AMALA
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Brief_description |
Species: human; Tissue: lymphocyte, B; Transformed by: Epstein-Barr
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Description |
Species: human female; Tissue: lymphocyte, B; Transformed by: Epstein-Barr
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Depositor |
Dr. M. P. Pistillo, IRCCS AOU San Martino - IST, Genoa, Italy
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Bibliographic_reference |
Tissue Antigens. 1996 Jun;47(6):538-46. [PMID: 8813743
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Morphology |
lymphoblast like, grown in suspension
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Culture_conditions |
continuous culture; RPMI 1640 + 10% FBS + 2mM L-Glutamine; Split 1:2-1:3 twice weekly. 37C, 5% CO2
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Viruses |
Search for viruses was not performed
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Release_conditions |
Cell line available for distribution. For non-commercial investigative use only
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Hazard |
Handle under Biosafety Level 2 containment
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Passage_number |
> 20
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Freezing_medium |
95% FCS + 5% DMSO
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Sterility |
mycoplasma negative, HOECHST and PCR
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Validation_assays |
HLA typing: A2; Cw9; B62; Bw6; DR14; DRw52; DQ7. A*2171; Cw*0303; B*1501; DRA*0102; DRB1*01402; DRB3*0101; DQA1*0503; DQB1*03011; DPA1*01; DPB1*0402
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Comments |
Originator of cell line: Prof. Z. Layrisse. Cell line from the 10th International Histocompatibility Workshop Cell Panel (10IHW).
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